Introduction:

    Xiaohong Fang received her B. S. degree in chemistry from Wuhan University in 1990 and obtained her M. S. and Ph. D. degrees from Peking University in 1993 and 1996, respectively. She went to University of Waterloo, Canada, in 1997 and did a one-year postdoctoral work there. During 1998-2001, she worked as a research scientist at Chemistry Department and Center for Research at Bio/nano Interface at University of Florida, USA. She was recruited to the Chinese Academy of Sciences in the “Hundred Distinguished Young Scholars Program” and was appointed as a chemistry professor in the Institute of Chemistry in December of 2001. She has been working in the field of bioanalytical and biophysical analysis and has over 100 publications in the well-known international journals.


Academic and Professional Awards:

Distinguished Young Scientists Award, China Association for Instrumental Analysis, 2008.

National Distinguished Scholars in the New Century, 2006.

Distinguished Women Award, Chinese Academy of Sciences, 2005.

"Hundred Distinguished Young Scholars" Achievement Award, Chinese Academy of Sciences, 2005.

Distinguished Young Scientists Award , Chinese Chemical Society, 2003

Chinese National Science Fund for Distinguished Young Scholars, Chinese National Science Foundation, 2002.

"Hundred Distinguished Young Scholars", Chinese Academy of Sciences, 2001

Research interest:

    The development and application of advanced bioanalytical and biophysical technologies and methodologies for the analysis of biologically important compounds and for the study of biomolecular interaction and reaction at the single molecule level. These include:
    (1) Single molecule Imaging and monitoring in living cell
    (2) Biomolecule characterization and interaction study by SPM
    (3) Nanobiosenor development
    (4) Novel molecular probes for protein recognition and real-time detection

Representative publication:

Single-Molecule Detection of Proteins Using Aptamer-Functionalized Molecular Electronic Devices, Liu, S.; Zhang, X., Luo, W., Wang, Z.., Guo, X.*, Steigerwald, M., Fang, X.*, Angew. Chem. Int. Ed. 2011,DOI: 10.1002/anie.201006469

A single-molecule study of inhibition effect of Naringenin on transforming growth factor-b ligand-receptor binding, Yang, Y., Xu, Y., Xia, T.,Chen, F., Zhang, F., Liang, W., Fang, X.* , Chem. Commun. 2011, 47, 2982-2984

A Water-Soluble C₆₀-Porphyrin Compound for Highly Efficient DNA Photocleavage, Zhou, C.*, Liu, Q., Xu, W., Wang, C. and Fang, X.*, Chem. Commun., 2011, 47, 2982-2984

Elasticity of Cardiac Cells on the Polymer Substrates with Different Stiffness: An Atomic Force Microscopy Study, Shi, X.,  Qin, L., Zhang, X., He, K., Xiong, C., Fang, J., Fang, X.*, Zhang, Y.*, Phys. Chem. Chem. Phys., 2011, 13 (16), 7540-5.

Single-Molecule Imaging Revealed Enhanced Dimerization of Transforming Growth Factor β Type Ⅱ Receptors in Hypertrophic Cardiomyocytes” He, K., Fu, Y., Zhang, W., Yuan, J., Li, Z., Lv, Z., Zhang, Y*, Fang, X.* Biochem. Biophy. Res. Commun., 2011,407，313-317.

Single-molecule fluorescence imaging of membrane-bound proteins for studies of cell signaling transduction，Yang, Y., Xia, T., Zhang, W., Fang, X.* Chinese Science bulletin, 2011, 56(11), 1063-67.^，

TGF-β signalling is mediated by two autonomously functioning TβRI:TβRII pairs. Huang T, David L, Mendoza V, Yang Y, Villarreal M, De K, Sun L, Fang X, López-Casillas F, Wrana JL, Hinck AP，EMBO J. 2011，30(7): 1263-76.

Aptamers Generated from Cell-SELEX for Molecular Medicine：A Chemical Biology Approach, Fang, X.* and Tan, W*, Acc. Chem. Res., 2010, 43, 48-57.

Intercellular transportation of quantum dots mediated by membrane nanotubes, He, K., Luo, W., Zhang, Y., Liu, F., Liu, D., Xu, L., Qin, L., Xiaong, C., Lu, Z., Fang, X.*, Zhang, Y.*, ACS Nano, 2010, 4，3015-3022。

Study of the inhibitory effect of water-soluble fullerenes on plant growth at the cellular level. Liu, Q., Zhao, Y., Wan, Y., Zhang, J., Zhang, X., Wang, C., Fang, X. * and Lin., J.* , ACS Nano, 2010, 4，5743-5748.

Monomeric Type I and Type III Transforming Growth Factor-β Receptors and Their Dimerization, Zhang, Z., Yuan, J., Yang, Y., Xu, L., Wang, Q., Fang, X.*, Chen, Y.*,  Cell Res., 2010. 20，1216-1223.

 Zhang, Z., Yuan, J., Yang, Y., Xu, L., Wang, Q., Fang, X.*, Chen, Y.*,  Cell Res., 2010. 20，1216-1223.

Study of the effect of atorvastatin on the interaction between ICAM-1 and CD11b by live-cell single-molecule force spectroscopy, Li, Y， Shi, X., Liu, H., Yi, S., Zhang, X., Fang, X.*, Science China: Chemistry, 2010, 53: 752-758.

Single-Molecule Imaging Reveals Transforming Growth Factor-b-induced Type II Receptor Dimerization. Zhang, W., Jiang, Y., Wang , Q., Ma, X., Xiao, Z., Zuo, W., Fang, X.*, Chen, Y.* Proc. Natl. Acad. Sci. USA, 2009, 106, 15679-15683.

Carbon Nanotubes as Molecular Transporters for Walled Plant Cells, Liu, Q., Bo. C., Wang, Q., Shi, X., Xiao, Z., Lin., J., and Fang, X. *, Nano Letters, 2009, 9: 1007-1010.

Study of inhibition effect of herceptin on interaction between heregulin and ErbB receptors HER3/HER2 by single-molecule force spectroscopy, Shi, X., Xu, L., Yu., J., and Fang, X. *, Exp. Cell. Res. 2009.

Molecular engineering of DNA: molecular beacons.K. Wang*, Z. Tang, C. Yang, Y. M. Kim, X. Fang, W. Li, Y. Wu, C. Medley, Z. Cao, J. Li, P. Colon, H. Lin, W. Tan*. Angew. Chem. Int. Ed., 2009, 48, 856-870.

Hydrodynamically Focused Stream as a Dynamic Template for Site-Specific Electrochemical Micropatterning of Conducting Polymers, Hou, S., Wang, S., Zeta, T., Yu, F., Nicole, Q., Zhu, M., Clifton, K,. Shen, F., Fang, X. *, Tseng, H.*, Angew. Chem. Int. Ed. 2008, 47,1072-1075

Single-Molecule Study of Lateral Mobility of Epidermal Growth Factor Receptor 2/HER2 on Activation，Z. Xiao, X. Ma, Y. Jiang, Z. Zhao, B. Lai, J. Liao, J. Yue and X. Fang*，J. Phy. Chem. B, 2008, 112: 4140-4145.

Detection of trace Hg²⁺ via induced circular dichroism of DNA wrapped around single-walled carbon nanotubes. X. Gao, G. Xing, Y. Yang, X. Shi, R. Liu, W. Chu, L. Jing, F. Zhao, C. Ye, H. Yuan, X. Fang, C. Wang, and Y. Zhao. J. Am. Chem. Soc., 2008, 130: 9190–9191.

TINY, a dehydration-responsive element(DRE)-binding protein-like transcription factor connecting the DRE-and ethylene-responsive element-mediated signaling patheways in Arabidopsis. S. Sun, J. Yu, F. Chen, T. Zhao, X. Fang, Y. Li, and S. Sui. J. Biol. Chem. 2008, 283: 6261-6271.

Single-Molecule Study of EGFR Mobility Implicates the its Association with Caveolae/Clathrin After Activation，Z. Xiao, W. Zhang, Y. Yang, L. Xu, X. Fang* Biochem Biophys Res Commun. 2008, 369: 730-734.

Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent， Shu，C., Ma, X., Zhang, J., Corwin, F., Sim, J., Zhang, E., Dorn, H., Gibson, H., Fatouros, P., Wang, C.*, Fang, X. * Bioconjugate Chem., 2008, 19: 651-655.

Cell-specific  Internalization  study of an Aptamer From  Whole Cell Selection, Xiao, Z., Shangguan, D., Cao, Z., Fang, X. and Tan, W. *, Chem. Eur. J., 2008, 14, 1679-1755.

Single Molecule Force Spectroscopy Study of Interaction between Transforming Growth Factor B and its Receptor in Living Cells，Yu., J., Wang Q., Shi X. , Ma, X., Yang H., Chen, Y., Fang, X*, J. J. Phy. Chem. B, 2007, 111(48):13619-13625

Lateral Diffusion of TGF-b Type Receptor Studied by Single Molecule Imaging, Ma, X., Wang,Q., Jiang, Y., Xiao, Z., Fang, X. *, Chen, Y*., Biochem Biophys Res Commun. 2007, 292, 31-40.

Interaction between Single Molecules of Mac-1 and ICAM-1 in Living Cells: An Atomic Force Microscopy Study, Yang, H., Yu, J. Fu, G., Shi, X., Xiao, L., Chen, Y., Fang, X*., Chen, H*., Exp. Cell. Res. 2007, 331, 3497-3504

Energy Landscape of Aptamer/Protein Complex Studied by Single Molecular Force Spectroscopy, Yu., J., Jiang, Y., Ma, X., Fang, X. * Chem. Asia. J., 2007, 2, 284-289.

Fluorescence study of DNA binding and bending by EcoRI DNA methyltransferase, Ma，B., Wang, J., Fang, X. *, J. Phy. Chem. B, 2006,110, 19647-19651.

Single Molecule Study of Binding Force Between Transcription Factor TINY and Its DNA Responsive Element, Yu, J., Sun, S., Jiang, Y., Ma, X., Chen, F., Zhang, G., Fang, X*., Polymer, 2006, 47, 2533-2538.

Detection of Oncoprotein Platelet-Derived Growth Factor Using Fluorescent Signaling Complex of Aptamer and TOTO，Zhou, C. Wang, J., Jiang, Y., Hou, S., Fang, X. *, Anal. Bioanal. Chem., 2006, 384, 1175-1180.

Aptamer Based ATP Assay Using a Luminescent Light Switching Complex, Wang, J., Jiang, Y., Zhou, C., Fang, X *. Anal. Chem., 2005, 77,3542-3546. (5.6)

Study of Fibrinogen Adsorption on Self-Assembled Monolayers on Au(111) by Atomic Force Microscopy，Lin ,Y., Wang, J., Wan L. , Fang X. *, Ultramicroscopy, 2005, 105, 129-136.

Study of the Single Molecular Interaction of the Transcription Factor ZmDREB1A with its DNA Responsive Element by Atomic Force Microscopy. Jiang, Y. X., Feng, Q., Fang, X*, Bai, C *. Nucl.Acid.Res. 2004, 32(12): e101. （7.6）

Signaling Aptamer/Protein Binding by a  Molecular Light-Switch Complex. Jiang, Y., Fang, X *, Bai, C *. Anal. Chem. 2004, 76, 5230-5235.

Delaying Photobleaching and Recovering Luminescence of a DNA Molecular Light Switch in DNA Analysis, Ling, L., Jiang, Y., Wang, C., Fang, X.*, Wan, L. He, Z., Bai, C.*, Chen.,D.*, Anal. Biochem. 2004, 329, 334-336.

Study the Effect of Metal Ion on the Specific Interaction between Protein and Aptamer by Atomic Force Microscopy, Jiang, Y. X., Wang, J., Fang, X., Bai, C. L., J. Nanosci. Nanotech., 2004, 4(6), 611-615.

Electrochemical Scanning Tunneling Microscope Imaging of Self-Assembly Monolayer of dsDNA on Au(111), Yang, G. Z., Wan, L.*, Fang, X*., Bai, C*. J. Nanosci. Nanotech., 2004, 4(6), 561-564.

Specific Aptamer - Protein Interaction Studied by Atomic Force Microscopy. Jiang, Y., Zhu, C. F., Ling, L., Wan, L., Fang, X *, Bai, C.*, Anal. Chem., 2003, 75(9): 2112-2116.

Study of Citrate Adsorbed on the Au(111) Surface by Scanning Probe Microscopy. Lin, Y., Pan, G.., Su, G. ., Fang, X.*, Wan, L.*., Bai, C.*. Langmuir 2003, 19, 10000-1003.